All functions |
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Precomputed lineage calling and coverage example. |
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Annotate diamond database hits with transcript info. |
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Annotate the sequences in the SILVA database file. |
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Convert a mbquant data table to a matrix. |
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Converts the denoise artifact to a phyloseq object. |
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Run differential association tests between taxa counts and exogenous factors. |
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Converts BRACKEN results to a phyloseq object. |
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Build the lineage from a SILVA path string. |
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Run differential association tests between between all combinations of a factor variable with DESeq2. Can be used as post-hoc test for regression. |
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Build a configuration for the alignment workflows. |
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Build a configuration for chimera removal. |
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Build a configuration for the transcript counting workflow. |
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Build a configuration for the demultiplexing workflow. |
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Build a configuration for the DADA2 workflow. |
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Build a configuration for the plate layout. |
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Build a configuration for power analysis. |
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Build a configuration for raw read preprocessing. |
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Build a configuration for the reference removal workflow. |
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Build a configuration for the SRA submission workflow. |
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Count alignment hits to a reference database. |
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Splits FASTQ files into individual samples. |
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Runs a full DADA2 workflow. |
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Discretize all continuous variables in a data frame. |
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Download the entire ENSEMBL transcript DB for bacteria. |
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Download a list of files. |
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Download data from SRA. |
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Calculate the effective transcript lengths. This is the mean number of positions in the transcript the fragment could map to. |
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Count transcripts using an Expectation Maximization (EM) algorithm. |
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Presets for read file formats. |
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Filter low presence taxa from a count matrix. |
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Filter a set of reference sequences from the data set.seed |
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Find alignment BAM files in a given directory. |
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Find read files in a given directory. |
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Checks whether taxa from one taxonomy table are contained in another table. |
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Generate a plate layout from a sample manifest. |
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Merge transcripts into a single database |
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miso. |
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Creates a plot of measured taxa quantifications vs. reference quantification. |
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Downloads a complete data set from mockrobiota. |
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Converts taxa annotations from mothur format to dada2 format. |
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Normalize a set of read counts across samples |
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Orlitsky's diminishing attenuation estimator (q2/3). |
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Parse annotations from an ENSEMBL id |
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Plot the Betabinomial fits for all taxa in a phyloseq object |
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Plots counts for several taxa across a co-variable |
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Plots the distribution of base entropy for each cycle. |
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Plots the distribution of sequence lengths with acceptable quality. |
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Plots the quality profile for an entire experiment. |
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Plots relative distribution for taxa across samples. |
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Run a power analysis for microbe abundances from a reference sample. |
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Runs preprocessing of sequencing reads. |
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Workflow for basic quality assessment of the read files. |
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Get the quality profiles and base calls for each cycle across all input files. |
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Read alignments from a BAM file. |
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Read a blast hit/alignment file. |
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Converts read count data to a phyloseq object. |
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Removes chimeric reads from amplicon sequencing data. |
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Removes sequence that map to a given reference. |
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Very simple select for miso artifacts. Just gets a particular entry. |
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Build the lineages for the entire SILVA database. |
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The available presets for SRA submissions. They all map to MIMARKS or MIMS packages |
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Prepare submission files for the NCBI sequence read archive (SRA). |
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Standardize all continuous columns of a data frame. |
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Counts the reads for a specific taxonomy level. |
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Calculates what percentage of taxa was found in a reference set. |
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Compares taxa quantification from a measurement to a reference ground truth. |
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Applies the specified types to a data frame-like object. |
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Quantify classification rates for each taxonomic rank. |